multimode fiber with a 600 μ m core diameter Search Results


multimode 8 atomic force microscope  (Bruker Corporation)
99
Bruker Corporation multimode 8 atomic force microscope
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Multimode 8 Atomic Force Microscope, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/MultiMode+8/pmc04625016-62-1-6
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fiber  (Thorlabs)
86
Thorlabs fiber
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Fiber, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/fiber/10__1016_slash_j__atech__2024__100603-54-7-8
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fiber - by Bioz Stars, 2026-08
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silica step index multimode fiber  (Thorlabs)
86
Thorlabs silica step index multimode fiber
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Silica Step Index Multimode Fiber, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/index+mmf+step/pmc12943972-55-8-20
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ocean hr2  (Ocean Optics)
95
Ocean Optics ocean hr2
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Ocean Hr2, supplied by Ocean Optics, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/Ocean+HR2/custom%40hr-2%4010%2E1021%2Facsomega%2E5c00293
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ocean hr6  (Ocean Optics)
99
Ocean Optics ocean hr6
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Ocean Hr6, supplied by Ocean Optics, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/Ocean+HR6/custom%40hr-6%4010%2E1021%2Facsomega%2E5c00293
Average 99 stars, based on 1 article reviews
ocean hr6 - by Bioz Stars, 2026-08
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fp600urt  (Thorlabs)
86
Thorlabs fp600urt
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Fp600urt, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/fp600ert/pm41755070-66-19-20
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multimode optical fibers  (Thorlabs)
86
Thorlabs multimode optical fibers
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Multimode Optical Fibers, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/fiber+multimode/10__1364_slash_boe__540470-82-0-9
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multimode optical fiber  (Avantes Inc)
86
Avantes Inc multimode optical fiber
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Multimode Optical Fiber, supplied by Avantes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/fiber+multimode+optical/pmc12898446-93-20-24
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lens  (Avantes Inc)
86
Avantes Inc lens
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Lens, supplied by Avantes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multimode+fiber+with+a+600+%CE%BC+m+core+diameter/lens/10__1016_slash_j__atech__2024__100603-54-21-22
Average 86 stars, based on 1 article reviews
lens - by Bioz Stars, 2026-08
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multimode step index fibers  (FORT FIBRE OTTICHE S R L)
90
FORT FIBRE OTTICHE S R L multimode step index fibers
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Multimode Step Index Fibers, supplied by FORT FIBRE OTTICHE S R L, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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usb-4000 ccd spectrometer  (Ocean Insight)
90
Ocean Insight usb-4000 ccd spectrometer
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Usb 4000 Ccd Spectrometer, supplied by Ocean Insight, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fiber coupled led mwwhf2 light source  (Thorlabs)
86
Thorlabs fiber coupled led mwwhf2 light source
A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .
Fiber Coupled Led Mwwhf2 Light Source, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .

Journal: PLoS ONE

Article Title: CLL Exosomes Modulate the Transcriptome and Behaviour of Recipient Stromal Cells and Are Selectively Enriched in miR-202-3p

doi: 10.1371/journal.pone.0141429

Figure Lengend Snippet: A) Transmission electron microscopy (TEM) image of exosomes enriched from CLL cell culture medium after 48 hrs. (a) Exosomes are visible as small 50–100 nm vesicles with bi-layered membranes (scale bar: 100 nm). (b) Exosomes were immune-isolated using magnetic beads coated with anti-HLA antibody and ultrathin sections of resultant beads processed for image analysis. The representative TEM image shows a bead coated with exosomes (scale bar: 0.2 μ m). B ) AFM images of exosomes from a representative CLL case immobilized on a mica surface using Peakforce Tapping mode (Multimode 8, Bruker). A) Topographic image of exosomes. B ) DMT Modulus image C) Adhesion image: The exosomes appear as circular biconvex vesicular structures. The DMT Modulus and adhesion images of exosomes show explicit sub-structures at the centre of the vesicles (arrows). D) Schematic cross-section following the line in the indicated exosome in C). C) FACS analysis of B-cell antigens on CLL exosomes prepared from primary CLL cases by density ultracentrifugation and adsorbed onto 4 μ m aldehyde-sulphate latex beads and incubated with isotype control, anti-HLA-A, B, C or CD19 antibodies followed by FITC-conjugated secondary antibody. Mean fluorescence intensities are plotted (mean ± standard error of mean (S.E.M.) (n = 9)). D) Representative analysis showing surface expression of CD81, HLA-DR, CD37, and integrin α4 (ITGA4) in CLL derived exosomes coupled to aldehyde-sulphate latex beads. Binding of FITC-conjugated isotype controls are included for comparison. E) Immunoblot analysis of CLL derived exosomes: Lysates from CLL exosome were probed for abundance of HLA-DR, MHC, IgM, Gp96, Lyn kinase, TSG 101 and Calnexin. Images are representative of analyses of 3 cases. WCE-Whole cell extract .

Article Snippet: A Multimode 8 atomic force microscope (Bruker), equipped with a 160-μm J-scanner and oxide-sharpened Si 3 N 4 cantilevers (600μm, k = 0.4 N·m −1 ) was operated in peak force tapping mode for imaging.

Techniques: Transmission Assay, Electron Microscopy, Cell Culture, Isolation, Magnetic Beads, Incubation, Control, Fluorescence, Expressing, Derivative Assay, Binding Assay, Comparison, Western Blot